galM [2019-08-01 14:44:33]

aldose-1-epimerase

locus: BSU_18360

pI: 5.60

mw: 37.07 kDa

protein length: 325 aa

gene length: 978 bp

function: unknown

product: aldose-1-epimerase (mutarotase)

essential: no

synonyms: yoxA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.8|Utilization of galactose]

Gene

Coordinates: 1,999,849 2,000,826

The protein

Catalyzed reaction/ biological activity

• aldose-1-epimerase (mutarotase) [Pubmed|19758330]

Protein family

• aldose epimerase family (single member, according to UniProt)
• α-D-glucose --> β-D-glucose (according to UniProt)

Structure

• [PDB|3MWX]

[SW|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|993A863ED80BA5970C9145A35D407834B322C9DC|galM]-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]
  description: [Pubmed|9733705]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|9733705], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|10482513], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• BKE18360 ([gene|993A863ED80BA5970C9145A35D407834B322C9DC|galM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACTCAACCTCTCC, downstream forward: _UP4_TAGCCAATGATGAAGCGCAG
• BKK18360 ([gene|993A863ED80BA5970C9145A35D407834B322C9DC|galM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTACTCAACCTCTCC, downstream forward: _UP4_TAGCCAATGATGAAGCGCAG

References

• 9733705,10482513,19758330